Bacteriophage T4 Genome

Bacteriophage T4 genome.

Phage T4 has provided countless contributions to the paradigms of genetics and biochemistry. Its complete genome sequence of 168,903 bp encodes about 300 gene products. T4 biology and its genomic sequence provide the best-understood model for modern functional genomics and proteomics. Variations on gene expression, including overlapping genes, internal translation initiation, spliced genes, tra...

The genome of bacteriophage T4.

Intra-strand biases in bacteriophage T4 genome.

In bacteriophage T4, a major portion of DNA replication is initiated at random along the map, although several proven and putative origins have been described for early replication. In order to analyze the contribution of transcription and translation as well as DNA replication to intra-strand bias from A = T and G = C, we examined the pattern of the intra-strand biases in the first, second, an...

The genome of bacteriophage T4: an archeological dig.

I was asked to share my reflections about an article safekeeping. I then looked at my old notebooks from the 1950s and 1960s—loose-leaf binders with the pages that appeared over 40 years ago in the 1963 Cold falling out, many undated, just lists of numbers. They Spring Harbor Symposium for Quantitative Biology. The artiwere total gibberish to me. I hid them away then and cle (Epstein et al. 196...

Integration of plasmids into the bacteriophage T4 genome.

We have analyzed the integration of plasmids into the bacteriophage T4 genome via homologous recombination. As judged by genetic selection for a plasmid-borne marker, a mutation in phage gene uvsX or uvsY essentially blocked the integration of a plasmid with homology to the T4 genome but no phage replication origin (non-origin plasmid). The strict requirement for these two proteins suggests tha...